Data is from one of two independent experiments. each, and infected WT and KO, n = 6 each respectively.(PDF) pone.0211244.s002.pdf (106K) GUID:?76CE694A-8D3E-4174-BBC5-BABBB173B104 S3 Fig: Cellularity of TM infected Mina WT and KO. The total cellularity of TM infected Mina WT and KO were assessed. The total numbers of CD19+ and CD11c+DC are assessed by flow cytometry PRKAR2 and plotted. Statistical analysis was carried out by Mann-Whitney test. There were no significant difference between the groups (WT and KO, n = 5 each for total cellularity and CD11cDC and for CD19+ cells WT n = 5; KO n = 3).(PDF) pone.0211244.s003.pdf (110K) GUID:?222BF76D-8920-46DF-BFAE-0B6A87BEAD52 S4 Fig: Proliferation of Mina KO T and B cells. CD4+CD25?CD45RBhi T cells (A and B) and CD19+ B cells (C and D) isolated from combined lymph node and spleen of Mina KO or WT littermate control mice were stimulated, respectively, with plate-bound anti-CD3/soluble anti-CD28, CD3/CD28 Dynabeads, LPS and anti-IgM respectively. Data are mean SEM (n = 6 mice). Log(mitogen concentration) versus cpm curves were fitted using a 4 parameter logistic curve model and EC50s of fitted curves were compared to determine statistical significance.(PDF) pone.0211244.s004.pdf (154K) GUID:?4B367BD2-F972-4439-8E23-D51368AE0773 S5 Fig: Histology of cecum from TM infected Mina WT and KO mice. Cecum from uninfected and Trichuris muris infected WT and Mina KO mice were harvested at d21 post infection. (A)The tissues were assessed for inflammation severity by hematoxylin eosin staining as described in the methods and (B) data from the histological assessment is shown. Data are from two independent experiments (WT and KO n = 13).(PDF) pone.0211244.s005.pdf (241K) Evista (Raloxifene HCl) GUID:?B22E0147-008C-4143-AAE1-EECDFC4F0742 S6 Fig: Serum IgE response to TM in Mina KO mice. Total serum IgE level from naive and TM infected WT and Mina KO mice at d21 p.i. The mice were infected by orogastric gavage with 150 TM embryonated eggs. Data are mean SD (Na?ve WT n = 6, and KO n = 4, TM infected WT n = 9, and KO n = 13 mice) from 2 independent experiments). Statistical significance was computed by the two-tailed Students t-test.(PDF) pone.0211244.s006.pdf (155K) GUID:?DD6CC095-9608-40B2-9FBD-9E6F47623FC3 S7 Fig: Th2 and Th17 response to TM in Mina KO mice. (A) Quantitative RT-PCR analysis of TSLP and IL33 mRNA in IECs from Mina KO and Evista (Raloxifene HCl) WT 21 dpi. Data are mean SD of n = 6 each of WT and KO for TSLP and WT n = 10, KO = 9 mice Evista (Raloxifene HCl) for IL33 from 2 independent experiments. TSLP ELISA was carried out on TM antigen stimulated mesenteric LN culture supernatants using a TSLP ELISA kit as described in methods. Data is from one of two independent experiments. (C) Cytokine analysis was performed for assessing IL17, IL13, IL4, IL10 using Milliplex cytokine analysis kit. Data are mean SEM (IL17; WT n = 8 and KO n = 10, IL13, WT n = 7 and KO n = 8, IL4; WT n = Evista (Raloxifene HCl) 9 and KO n = 7; IL10; WT n = 9 and KO = 10 mice; from 2 independent experiments). Statistical significance was computed by the two-tailed Students t-test.(PDF) pone.0211244.s007.pdf (168K) GUID:?D836F339-8C34-4742-B695-89FA5C9E87CE S8 Fig: In vitro differentiation of Mina KO CD4 T cells. Mina KO and WT littermate controls were differentiated under Th1 and Th2 conditions as described in methods. Shown are the mean SD (n = 3 mice for Th1 and n = 4 for Th2 from 1 of 2 representative experiments). Statistical significance was computed by the two-tailed Students t-test.(PDF) pone.0211244.s008.pdf (160K) GUID:?084EDAA1-31F3-4034-AA70-759B4C7D9887 S9 Fig: Splenic and IEC Mina expression in MinaIEC mice. Mina mRNA expression level in splenocytes and IECs from MinaIEC [VillinCre+::Mina(fl/fl)] and controls VillinCre+::Mina(+/+) and VillinCre-::Mina(fl/fl)] mice. Shown are the mean SEM (n = 11, 5 and 9 for Splenocytes and n = 7, 5, 8 for IECs respectively from 2 independent experiments). Statistical significance was computed by the two-tailed Students t-test.(PDF) pone.0211244.s009.pdf (149K) GUID:?2238B369-EEE6-4BFF-9A4B-CE65C2A06A87 S10 Fig: Accelerated clearance of in the absence of Mina53. WT and Mina KO mice were infected by subcutaneous injection with 500 L3 larvae. At 7 dpi the mice were sacrificed and intestines were collected, dissected and resident larvae and adult worms were counted using a dissection microscope. Data are mean SD (n = 6 mice from one experiment). Statistical significance was computed Evista (Raloxifene HCl) by the Mann-Whitney test.(PDF) pone.0211244.s010.pdf (166K) GUID:?E26C5D5D-FE02-489E-9F24-9966E01C4517 S11 Fig: Quantitative RT-PCR analysis of Rbp2. Quantitative RT-PCR analysis of Rbp2 in 21 d.p.i. Mina KO and WT IECs. Data are mean SD (WT n = 8, and KO n = 5 mice from 2 independent experiments). Statistical significance was computed by the two-tailed Students t-test.(PDF) pone.0211244.s011.pdf (173K) GUID:?01327EAA-83ED-46C8-8E04-CC3D6770B249 S12 Fig: Cytotoxic activity of -defensins toward L3 TM larvae. Cytotoxic activity of reduced (r-) -defensins 5, 20, 21, 23 and 24 against.